DEL Decoding

DELi support decoding of raw DEL selection data (DNA sequences, or “reads”) into DEL compound enrichment data (what scientist and the analysis module use to find “hits”). This what the decode module in DELi handles.

• Decoding Algorithm
  • Library Demultiplexing
  • Barcode Calling
  • Barcode Decoding
  • UMI extraction
  • Decode Parallelization and Collection
  • UMI Degeneration
  • References
• Barcode Calling Hashmaps
  • Error Correction
• Decoding Outcomes
  • Failed Decode Outcomes
• Tool Compounds
  • Decoding Tool Compounds
  • Doping Tool Compounds
  • Adding Tool Compounds
  • How DELi handles tool compounds
• Running DELi Decode
  • What you need to run a decoding job
  • Running a decode job
  • Decoding job outputs
  • Collecting decoding runs
  • Counting UMIs
• Decoding Settings